期刊
STEM CELLS AND DEVELOPMENT
卷 22, 期 19, 页码 2619-2629出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/scd.2013.0043
关键词
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资金
- Deutsche Forschungsgemeinschaft (DFG) [SCHR992/3-1, SCHR992/4-1]
- ISHLT Shumway Career Development Award
- ISHLT
Mesenchymal stromal cells (MSCs) have been isolated from many tissues, including gestational tissue. To date, a study comparing the properties and suitability of these cells in cell-based therapies is lacking. In this study, we compared the phenotype, proliferation rate, migration, immunogenicity, and immunomodulatory capabilities of human MSCs derived from umbilical cord lining (CL-MSCs), umbilical cord blood (CB-MSCs), placenta (P-MSCs), and Wharton's jelly (WJ-MSCs). Differences were noted in differentiation, proliferation, and migration, with CL-MSCs showing the highest proliferation and migration rates resulting in prolonged survival in immunodeficient mice. Moreover, CL-MSCs showed a prolongation in survival in xenogeneic BALB/c mice, which was attributed to their ability to dampen T(H)1 and T(H)2 responses. Weaker human cellular immune responses were detected against CL-MSCs and P-MSCs, which were correlated with their lower HLA I expression. Furthermore, HLA II was upregulated less substantially by CL-MSCs and CB-MSCs after IFN- stimulation. MSC types did not differ in indolamine 2,3-dioxygenase (IDO) expression after IFN- stimulation. Despite their lower IDO, HLA-G, and TGF-1 expression, only CL-MSCs were able to reduce the release of IFN- by lymphocytes in a mixed lymphocyte reaction. In summary, CL-MSCs showed the best characteristics for cell-based strategies, as they are hypo-immunogenic and show high proliferation and migration rates. In addition, these studies show for the first time that although immunomodulatory molecules HLA-G, HLA-E, and TGF- play an important role in MSC immune evasion, basal and induced HLA expression seems to be decisive in determining the immunogenicity of MSCs.
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