期刊
STEM CELLS AND DEVELOPMENT
卷 22, 期 7, 页码 1063-1075出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/scd.2012.0315
关键词
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资金
- La Fondation CHU Sainte-Justine
- La Fondation Charles-Bruneau
- FRQS, Fonds de la Recherche en Sante du Quebec
- Pediatric Immunodeficiency Treatment Consortium (PIDTC) [U54AI082973]
The mechanisms by which mesenchymal stromal cells (MSCs) induce immunomodulation are still poorly understood. In the current work, we show by a combination of polymerase chain reaction (PCR) array, flow cytometry, and multiplex cytokine data analysis that during the inhibition of an alloantigen-driven CD4(+) T-cell response, MSCs induce a fraction of CD4(+) T-cells to coexpress interferon-gamma (IFN gamma) and interleukin-10 (IL-10). This CD4(+) IFN gamma(+) IL-10(+) cell population shares properties with recently described T-cells originating from switched Th1 cells that start producing IL-10 and acquire a regulatory function. Here we report that IL-10-producing Th1 cells accumulated with time during T-cell stimulation in the presence of MSCs. Moreover, MSCs caused stimulated T-cells to downregulate the IFN gamma receptor (IFN gamma R) without affecting IL-10 receptor expression. Further, the inhibitory effect of MSCs could be reversed by an anti-IFN gamma R-blocking antibody, indicating that IFN gamma is one of the major players in MSC-induced T-cell suppression. Stimulated (and, to a lesser extent, resting) CD4(+) T-cells treated with MSCs were able to inhibit the proliferation of autologous CD4(+) T-cells, demonstrating their acquired regulatory properties. Altogether, our results suggest that the generation of IL-10-producing Th1 cells is one of the mechanisms by which MSCs can downmodulate an immune response.
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