4.5 Article

Amniotic Fluid Stem Cells Are Cardioprotective Following Acute Myocardial Infarction

期刊

STEM CELLS AND DEVELOPMENT
卷 20, 期 11, 页码 1985-1994

出版社

MARY ANN LIEBERT, INC
DOI: 10.1089/scd.2010.0424

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资金

  1. British Heart Foundation
  2. Engineering and Physical Sciences Research Council
  3. Biotechnology and Biological Sciences Research Council
  4. Citta Della Speranza, Malo (VI), Italy
  5. Great Ormond Street Hospital Children's Charity, United Kingdom
  6. British Heart Foundation [FS/08/004/23625] Funding Source: researchfish
  7. Engineering and Physical Sciences Research Council [EP/I014667/1] Funding Source: researchfish
  8. Great Ormond Street Hospital Childrens Charity [V1230] Funding Source: researchfish
  9. EPSRC [EP/I014667/1] Funding Source: UKRI

向作者/读者索取更多资源

In recent years, various types of stem cells have been characterized and their potential for cardiac regeneration has been investigated. We have previously described the isolation of broadly multipotent cells from amniotic fluid, defined as amniotic fluid stem (AFS) cells. The aim of this study was to investigate the therapeutic potential of human AFS cells (hAFS) in a model of acute myocardial infarction. Wistar rats underwent 30 min of ischemia by ligation of the left anterior descending coronary artery, followed by administration of hAFS cells and 2 h of reperfusion. Infarct size was assessed by 2,3,5-triphenyltetrazolium chloride staining and planimetry. hAFS cells were also analyzed by enzyme-linked immunosorbent assay to detect secretion of putative paracrine factors, such as the actin monomer-binding protein thymosin beta 4 (T beta 4). The systemic injection of hAFS cells and their conditioned medium (hAFS-CM) was cardioprotective, improving myocardial cell survival and decreasing the infarct size from 53.9%+/- 2.3% (control animals receiving phosphate-buffered saline injection) to 40.0%+/- 3.0% (hAFS cells) and 39.7%+/- 2.5% (hAFS-CM, P < 0.01). In addition, hAFS cells were demonstrated to secrete T beta 4, previously shown to be both cardioprotective and proangiogenic. Our results suggest that AFS cells have therapeutic potential in the setting of acute myocardial infarction, which may be mediated through paracrine effectors such as T beta 4. Therefore, AFS cells might represent a novel source for cell therapy and cell transplantation strategies in repair following ischemic heart disease, with a possible paracrine mechanism of action and a potential molecular candidate for acute cardioprotection.

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