期刊
STEM CELLS AND DEVELOPMENT
卷 17, 期 3, 页码 475-482出版社
MARY ANN LIEBERT INC
DOI: 10.1089/scd.2007.0120
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Although dental pulp stem cells (DPSC) have been isolated from adult dental pulp tissues, knowledge on how to use them to make teeth lags behind. To date, little is known about the effects of epithelial-mesenchymal cell ratios on the bioengineered odontogenesis mediated by DPSCs. In this study, we investigated the effects of apical bud cells (ABC) from dental epithelial stem cell niche of rat incisors on the differentiation and morphogenesis of molar DPSCs at different proportions (DPSC/ABC cell ratios = 1: 10, 1: 3, 1: 1, 3: 1, 10: 1, respectively). In vitro mixed DPSCs/ABCs at 1: 1, 1: 3, and 3: 1 ratios displayed several crucial characteristics of odontoblast/ameloblast lineages, as indicated by accelerated mineralization, upregulated alkaline phosphatase activity, protein/gene expression for dentin sialophosphoprotein and ameloblastin. In vivo transplantation of reassociated DPSC and ABC pellets at different ratios was also carried out. Histological analyses demonstrated that only DPSC/ABC recombinants at 1: 1 ratio generated typical molar crown-shaped structures, whereas recombinations at other ratios presented an atypical crown morphogenesis with unbalanced distribution of amelogenesis and dentinogenesis. Together, these findings revealed that the proportions of dental epithelial and mesenchymal cell populations can determine the odontogenic differentiation of DPSCs/ABCs in vitro as well as the bioengineered tooth morphogenesis in vivo.
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