期刊
STEM CELLS
卷 31, 期 9, 页码 2024-2030出版社
WILEY
DOI: 10.1002/stem.1391
关键词
Tissue-specific stem cells; Cell surface markers; Fluorescence-activated cell sorting; Flow cytometry; Cell culture
资金
- NC TraCS Institute Pilot Grant Program [2KR271103, 2KR381202]
- Center for Gastrointestinal Biology and Disease [5P30DK034987-27]
- University Cancer Research Fund Innovation Award
- NIH National Center for Research Resources
- National Center for Advancing Translational Sciences, National Institutes of Health
Recent seminal studies have rapidly advanced the understanding of intestinal epithelial stem cell (IESC) biology in murine models. However, the lack of techniques suitable for isolation and subsequent downstream analysis of IESCs from human tissue has hindered the application of these findings toward the development of novel diagnostics and therapies with direct clinical relevance. This study demonstrates that the cluster of differentiation genes CD24 and CD44 are differentially expressed across LGR5 positive active stem cells as well as HOPX positive facultative stem cells. Fluorescence-activated cell sorting enables differential enrichment of LGR5 (CD24-/CD44+) and HOPX (CD24+/CD44+) cells for gene expression analysis and culture. These findings provide the fundamental methodology and basic cell surface signature necessary for isolating and studying intestinal stem cell populations in human physiology and disease. STEM Cells2013;31:2024-2030
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