期刊
STEM CELLS
卷 31, 期 1, 页码 92-103出版社
WILEY
DOI: 10.1002/stem.1267
关键词
Endothelial cell; Stem cells; Induced pluripotent stem cells; Cell differentiation
资金
- NHLBI/NIH [HL100406, HL098179]
- L.K. Whittier Foundation
- William Younger Family Foundation
- Eugene Roddenberry Foundation
- California Institute for Regenerative Medicine (CIRM)
- NIH/NCRR [C06RR018928]
- American Heart Association
- National University of Singapore
- National Institutes of Health [RC2HL103400, 1U01HL100397, U01HL099775]
- University of California [18XT-0098, 20FT-0090]
- Wallace H. Coulter Translational Research Grant Program
- Stanford Cardiovascular Institute
- NATIONAL CENTER FOR RESEARCH RESOURCES [C06RR018928] Funding Source: NIH RePORTER
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [U01HL100406, RC2HL103400, UM1HL098179, U01HL098179, U01HL099775, U01HL100397] Funding Source: NIH RePORTER
Recent evidence suggests human embryonic stem cell (hESC) and induced pluripotent stem (iPS) cell lines have differences in their epigenetic marks and transcriptomes, yet the impact of these differences on subsequent terminally differentiated cells is less well understood. Comparison of purified, homogeneous populations of somatic cells derived from multiple independent human iPS and ES lines will be required to address this critical question. Here, we report a differentiation protocol based on embryonic development that consistently yields large numbers of endothelial cells (ECs) derived from multiple hESCs or iPS cells. Mesoderm differentiation of embryoid bodies was maximized, and defined growth factors were used to generate KDR+ EC progenitors. Magnetic purification of a KDR+ progenitor subpopulation resulted in an expanding, homogeneous pool of ECs that expressed EC markers and had functional properties of ECs. Comparison of the transcriptomes revealed limited gene expression variability between multiple lines of human iPS-derived ECs or between lines of ES- and iPS-derived ECs. These results demonstrate a method to generate large numbers of pure human EC progenitors and differentiated ECs from pluripotent stem cells and suggest individual lineages derived from human iPS cells may have significantly less variance than their pluripotent founders. STEM CELLS 2013;31:92-103
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