期刊
STEM CELLS
卷 29, 期 3, 页码 549-553出版社
WILEY
DOI: 10.1002/stem.594
关键词
Reprogramming; iPSC; TGF-beta; Pluripotent stem cells
资金
- NICHD
- NHLBI
- NIMH/NIH
- California Institute for Regenerative Medicine
- Prostate Cancer Foundation
- Fate Therapeutics
- Esther B. O'Keeffe Foundation
- Scripps Research Institute
- National Science Foundation of China [90919060]
- China National Basic Research Program [2011CB965301]
It has been established that exogenous expression of four transcription factors (Oct4, Klf4, Sox2, and c-Myc) can reprogram mammalian somatic cells to pluripotent states. Further studies demonstrated that such induced pluripotent stem cells (iPSCs) could be generated with fewer exogenous transcription factors, facilitated by endogenous expression of reprogramming factors and/or synthetic small molecules. Here, we reported identification of a new small molecule, a protein arginine methyltransferase inhibitor AMI-5, which enabled Oct4-induced reprogramming of mouse embryonic fibroblasts in combination with transforming growth factor (TGF)-beta inhibitor A-83-01. The Oct4-induced iPSCs were shown similar to mouse embryonic stem cells with respect to typical pluripotency criteria. More importantly, they were shown to give rise to liveborn pups through tetraploid complementation assays, demonstrating the high quality of full reprogramming induced by this condition. Furthermore, this study suggests that regulation of protein arginine methylation might be involved in the reprogramming process. STEM CELLS 2011;29:549-553
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