期刊
STEM CELLS
卷 27, 期 5, 页码 1057-1065出版社
WILEY
DOI: 10.1002/stem.41
关键词
Human embryonic stem cells; CD30; Apoptosis; Chromosomal aberrations; Embryonal carcinoma; Culture adaptation
资金
- Medical Research Council
- Yorkshire Cancer Research
- Juvenile Diabetes Research Foundation International
- ESTOOLS
- BBSRC [BB/D524908/1] Funding Source: UKRI
- MRC [G0700785] Funding Source: UKRI
- Medical Research Council [G0700785] Funding Source: researchfish
Human embryonic stem cells undergo adaptive changes that can increase their growth capacity upon prolonged culture in vitro. This is frequently associated with nonrandom karyotypic changes, commonly involving amplification of genetic material from chromosomes 12, 17, and X. A recent study suggested that the karyotypically abnormal cells can be identified by their expression of CD30, which confers resistance to apoptosis. We have now investigated CD30 expression and apoptosis in karyotypically normal and abnormal sublines of the human ES cell line, H7, but our results were contrary to those previously observed. In this cell line, CD30 expression did not segregate the normal and abnormal cells, and abnormal cells were not protected from apoptosis. These data suggest that culture adaptation can occur through a variety of mechanisms. STEM CELLS 2009;27:1057-1065C
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