期刊
STEM CELLS
卷 27, 期 6, 页码 1318-1329出版社
WILEY
DOI: 10.1002/stem.52
关键词
Hematopoietic stem cell; Self-renewal; Wnt; Stroma; Stem cell-microenvironment interactions
资金
- Ministry of Health, Welfare and Family [0405-DB01-0104-0006]
- Korea Science and Engineering Foundation (KOSEF) [2008-05981]
- Korean Government (Mininstry of Education, Science, and Technology [MEST])
- Korea Basic Sicence Institute, MEST
With contrasting observations on the effects of beta-catenin on hematopoietic stem cells (HSCs), the precise role of Wnt/beta-catenin signals on HSC regulation remains unclear. Here, we show a distinct mode of Wnt/beta-catenin signal that can regulate HSCs in a stroma-dependent manner. Stabilization of beta-catenin in the bone marrow stromal cells promoted maintenance and self-renewal of HSCs in a contact-dependent manner, whereas direct stabilization in hematopoietic cells caused loss of HSCs. Interestingly, canonical Wnt receptors and beta-catenin accumulation were predominantly enriched in the stromal rather than the hematopoietic compartment of bone marrows. Moreover, the active form of beta-catenin accumulated selectively in the trabecular endosteum in Wnt 3a-stimulated or irradiationstressed, but not in steady- state marrows. Notably, notch ligands were induced in Wnt/beta-catenin activated bone marrow stroma and downstream notch signal activation was seen in the HSCs in contact with the activated stroma. Taken together, Wnt/beta-catenin activated stroma and their cross-talk with HSCs may function as a physiologically regulated microenvironmental cue for HSC self-renewal in the stem cell niche. STEM CELLS 2009;27:1318-1329
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据