期刊
STEM CELLS
卷 26, 期 9, 页码 2349-2360出版社
WILEY-BLACKWELL
DOI: 10.1634/stemcells.2008-0080
关键词
Parkinson's disease; adult neurogenesis; subventricular zone; striatum; transforming growth factor; neuroblasts
资金
- NIH/National Institute of Neurological Disorders and Stroke [P50-NS39793]
- Orchard Foundation
- Anti-Aging Foundation
- Michael K. Stern Foundation
- Harold and Ronna Cooper Family
- National Institute on Drug Abuse [K02-DA023555, R01-DA16765]
- National Alliance for Research on Schizophrenia and Depression Young Investigator Award
- Canadian Institute of Health Research
Infusion of transforming growth factor alpha (TGF alpha) into the adult dopamine (DA)-depleted striatum generates a local population of nestin(+)/proliferating cell nuclear antigen (PCNA)(+) newborn cells. The precise origin and fate of these new striatal cells are unknown, making it difficult to direct them for neural repair in Parkinson's disease. Experiments in rats using 5-bromo-2'-deoxyuridine (BrdU) to label neural progenitor cells showed that during TGF alpha infusion in the DA-depleted striatum, newborn striatal cells formed a homogeneous population of precursors, with the majority coexpressing nestin, Mash1, Olig2, and epidermal growth factor receptor, consistent with the phenotype of multipotent C cells. Upon TGF alpha pump withdrawal, the subventricular zone (SVZ) was repopulated by neuroblasts. Strikingly, during this period, numerous clusters of doublecortin(+)/polysialylated neuronal cell adhesion molecule(+) neuroblasts were also produced in the ipsilateral medial striatum. In parallel, striatal BrdU(+)/glial fibrillary acidic protein(+) astrocytes were generated, but no BrdU(+)/O4(+)/CNPase(+) oligodendrocytes were generated. Infusion of the neuralizing bone morphogenetic protein antagonist noggin after TGF alpha pump withdrawal increased the neuroblast-to-astrocyte ratio among new striatal cells by blocking glial differentiation but did not alter striatal neurogenesis. At no time or treatment condition were differentiated neurons generated, including DA neurons. Using 6-hydroxydopamine-lesioned nestin-CreERT2/R26R-YFP mice that allow genetic fate-mapping of SVZ nestin(+) cells, we show that TGF alpha-generated striatal cells originate from SVZ nestin(+) precursors that confirmed data from the rats on the phenotype and fate of striatal nestin(+)/PCNA(+) cells upon TGF alpha withdrawal. This work demonstrates that a large population of multipotent striatal C-like cells can be generated in the DA-depleted striatum that do not spontaneously differentiate into DA neurons.
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