期刊
STEM CELLS
卷 26, 期 9, 页码 2391-2398出版社
ALPHAMED PRESS
DOI: 10.1634/stemcells.2008-0043
关键词
human adipose stromal cells; xenotransplantation; muscular dystrophy; therapy
资金
- FAPESP-CEPID (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo-Centro de Pesquisa, Inovacao e Difusao)
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
Limb-girdle muscular dystrophies (LGMDs) are a heterogeneous group of disorders characterized by progressive degeneration of skeletal muscle caused by the absence of or defective muscular proteins. The murine model for limb-girdle muscular dystrophy 2B (LGMD2B), the SJL mice, carries a deletion in the dysferlin gene that causes a reduction in the protein levels to 15% of normal. The mice show muscle weakness that begins at 4-6 weeks and is nearly complete by 8 months of age. The possibility of restoring the defective muscle protein and improving muscular performance by cell therapy is a promising approach for the treatment of LGMDs or other forms of progressive muscular dystrophies. Here we have injected human adipose stromal cells (hASCs) into the SJL mice, without immunosuppression, aiming to assess their ability to engraft into recipient dystrophic muscle after systemic delivery; form chimeric human/mouse muscle fibers; express human muscle proteins in the dystrophic host and improve muscular performance. We show for the first time that hASCs are not rejected after systemic injection even without immunosuppression, are able to fuse with the host muscle, express a significant amount of human muscle proteins, and improve motor ability of injected animals. These results may have important applications for future therapy in patients with different forms of muscular dystrophies.
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