期刊
STEM CELLS
卷 26, 期 9, 页码 2321-2331出版社
ALPHAMED PRESS
DOI: 10.1634/stemcells.2007-1095
关键词
extracellular matrix; RNA binding protein; alternative splicing; signal transducer and activator of RNA; forebrain development
资金
- International Graduate School of Neuroscience
- Deutsche Forschungsgemeinschaft
- German Research Foundation [Ho-2476]
- Bundesministerium fur Bildung und Forschung [01GN0503]
Neural stem cells (NSCs) reside in a niche that abounds in extracellular matrix (ECM) molecules. The ECM glycoprotein tenascin-C (Tnc) that occurs in more than 25 isoforms represents a major constituent of the privileged NSC milieu. To understand its role for NSCs, the induction gene trap technology was successfully applied to mouse embryonic NSCs, and a library of more than 500 NSC lines with independent gene trap vector integrations was established. Our pilot screen identified Sam68 as a target of Tnc signaling in NSCs. The Tnc-mediated down-regulation of Sam68, which we found expressed at low levels in the niche along with Tnc, was independently confirmed on the protein level. Sam68 is a multifunctional RNA-binding protein, and its potential significance for cultured NSCs was studied by overexpression. Increased Sam68 levels caused a marked reduction in NSC cell proliferation. In addition, Sam68 is a signal-dependent regulator of alternative splicing, and its overexpression selectively increased the larger Tnc isoforms, whereas a mutated phosphorylation-deficient Sam68 variant did not. This emphasizes the importance of Sam68 for NSC biology and implicates an instructive rather than a purely permissive role for Tnc in the neural stem cell niche.
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