期刊
STEM CELL REVIEWS AND REPORTS
卷 11, 期 3, 页码 453-459出版社
SPRINGER
DOI: 10.1007/s12015-014-9561-9
关键词
Chondrogenesis; Adipose-derived stem cells; PLGA; Gelatin microspheres; Transforming growth factor-beta 1
Articular cartilage has restricted self-regenerative capacity; therefore, treatment of cartilage lesions is a great challenge in the field of orthopedics. In the present study, we evaluate the enhancing effect of a transforming growth factor-beta 1 (TGF-beta 1)-immobilized scaffold, fabricated by incorporating TGF-beta 1-loaded gelatin microspheres into PLGA framework, on the differentiation of adipose-derived stem cells (ASCs) into chondrocytes. Significant increase in cell proliferation was observed in the TGF-beta 1-immobilized PLGA-gelatin scaffold, as compared with the ASC-seeded non-TGF-beta 1-immobilized PLGA-gelatin scaffold. When chondrogenic differentiation of ASCs was evaluated for both constructs, sulfated glycosaminoglycan (sGAG) content was significantly higher in the TGF-beta 1-immobilized scaffold. This study showed that ASCs containing the TGF-beta 1-immobilized scaffold better promoted cartilage regeneration in defective articular cartilage, which is assessed by histological observation. Based on the above results, we conclude that TGF-beta 1-immobilized PLGA-gelatin scaffold seeded with ASCs considerably enhances the quality of the tissue-engineered cartilage, therefore, advancing the field of cartilage tissue engineering.
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