期刊
STEM CELL REVIEWS AND REPORTS
卷 7, 期 4, 页码 799-814出版社
SPRINGER
DOI: 10.1007/s12015-011-9255-5
关键词
Neural crest; Adult stem cell; Human epidermal neural crest stem cell; EPI-NCSC; hEPI-NCSC; Pluripotency; Bone; Melanocyte
资金
- Medical Research Council UK [22358]
- National Institute of Neurological Disease and Stroke, NIH, USA [R01 NS038500-04S1]
- North East England Stem Cell Institute, Newcastle University, Newcastle upon Tyne, UK
- Plunkett Family Foundation, Milwaukee, Wisconsin, USA
- MRC [G0802547] Funding Source: UKRI
- Medical Research Council [G0802547] Funding Source: researchfish
Here we describe the isolation, characterisation and ex-vivo expansion of human epidermal neural crest stem cells (hEPI-NCSC) and we provide protocols for their directed differentiation into osteocytes and melanocytes. hEPI-NCSC are neural crest-derived multipotent stem cells that persist into adulthood in the bulge of hair follicles. Multipotency and self-renewal were determined by in vitro clonal analyses. hEPI-NCSC generate all major neural crest derivatives, including bone/cartilage cells, neurons, Schwann cells, myofibroblasts and melanocytes. Furthermore, hEPI-NCSC express additional neural crest stem cell markers and global stem cell genes. To variable degrees and in a donor-dependent manner, hEPI-NCSC express the six essential pluripotency genes C-MYC, KLF4, SOX2, LIN28, OCT-4/POU5F1 and NANOG. hEPI-NCSC can be expanded ex vivo into millions of stem cells that remain mulitpotent and continue to express stem cell genes. The novelty of hEPI-NCSC lies in the combination of their highly desirable traits. hEPI-NCSC are embryonic remnants in a postnatal location, the bulge of hair follicles. Therefore they are readily accessible in the hairy skin by minimal invasive procedure. hEPI-NCSC are multipotent somatic stem cells that can be isolated reproducibly and with high yield. By taking advantage of their migratory ability, hEPI-NCSC can be isolated as a highly pure population of stem cells. hEPI-NCSC can undergo robust ex vivo expansion and directed differentiation. As somatic stem cells, hEPI-NCSC are conducive to autologous transplantation, which avoids graft rejection. Together, these traits make hEPI-NCSC novel and attractive candidates for future cell-based therapies and regenerative medicine.
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