期刊
STEM CELL REVIEWS AND REPORTS
卷 6, 期 3, 页码 390-397出版社
SPRINGER
DOI: 10.1007/s12015-010-9160-3
关键词
iPS cells; Tail tip fibroblast (TTF); Fully pluripotent; Tetraploid complementation; Viable mice
资金
- China National Basic Research Program [2006CB701500, 2007CB947800]
- Shanghai Leading Academic Discipline Project [S30201]
- STCSM Project [08dj1400502]
Previous studies demonstrated that induced pluripotent stem (iPS) cells could produce viable mice through tetraploid complementation, which was thought to be the most stringent test for pluripotency. However, these highly pluripotent iPS cells were previously reported to be generated from fibroblasts of embryonic origin. Achieving fully pluripotent iPS cells from multiple cell types, especially easily accessible adult tissues, will lead to a much greater clinical impact. We successfully generated high-pluripotency iPS cells from adult tail tip fibroblasts (TTF) that resulted in viable, full-term, fertile TTF-iPS animals with no obvious teratoma formation or other developmental abnormalities. Comparison of iPS cells from embryonic origin (MEF), progenitor cells (neural stem cells) or differentiated somatic cells (TTF) reveals that fully pluripotent developmental potential can be reached by each cell type, although with different induction efficiencies. This work provides the means for studying the mechanisms and regulation of direct reprogramming, and has encouraging implications for future clinical applications and therapeutic interventions.
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