期刊
STEM CELL RESEARCH
卷 4, 期 2, 页码 148-154出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scr.2009.12.001
关键词
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资金
- Swedish Research Council [11624]
- King Gustaf V's and Queen Victoria's foundation
- Swedish Heart and Lung Foundation
- Swedish Stroke Foundation
- Spjutspetsprojekt at the County of Vasterbotten
- Northern Sweden Stroke Foundation
- Medical Faculty of Umea University, Umea University Hospital, Umea, Sweden
- Swedish Society for Medicine
Neurogenesis occurs in the cerebral cortex of adult rats after focal cerebral ischemia. Whether or not the newborn neurons could synthesize neurotransmitters is unknown. To elucidate such a possibility, a photothrombotic ring stroke model with spontaneous reperfusion was induced in adult male Wistar rats. The DNA duplication marker BrdU was repeatedly injected, and the rats were sacrificed at various times after stroke. To detect BrdU nuclear incorporation and various neurotransmitters, brain sections were processed for single/double immunocytochemistry and single/double/triple immunofluorescence. Stereological cell counting was performed to assess the final cell populations. At 48 h, 5 days, 7 days, 30 days, 60 days and 90 days after stroke, numerous cells were BrdU-immunolabeled in the penumbral cortex. Some of these were doubly immunopositive to the cholinergic neuron-specific marker ChAT or GABAergic neuron-specific marker GAD. As analyzed by 3-D confocal microscopy, the neurotransmitters acetylcholine and GABA were colocalized with BrdU in the same cortical cells. In addition, GABA was colocalized with the neuron-specific marker Neu N in the BrdU triple-immunolabeled cortical cells. This study suggests that the newborn neurons are capable of synthesizing the neurotransmitters acetylcholine and GABA in the penumbral cortex, which is one of the fundamental requisites for these neurons to function in the poststroke recovery. (C) 2009 Elsevier B.V. All rights reserved.
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