期刊
SPECTROSCOPY-AN INTERNATIONAL JOURNAL
卷 27, 期 2, 页码 93-105出版社
HINDAWI LTD
DOI: 10.1155/2012/276795
关键词
Water content; VIS/NIR spectroscopy; PLS; wavelength selection
资金
- National Natural Science Foundation [60708026]
- Beijing Municipal Training Program Foundation for the Excellent Talents [20081D1600600348]
- Programs for Changjiang Scholars and Innovative Research Team (PCSIRT) in University of China [IRT0705]
Water content in plants is one of the most common biochemical parameters limiting efficiency of photosynthesis and crop productivity. Therefore, it has very important meaning to predict the water content rapidly and nondestructively. The objective of this study was to investigate the feasibility of detecting the water content in the leaf using the diffuse reflectance spectra limited in the VIS/NIR region (400-1100 nm), which could be used to determine other biochemical parameters such as chlorophyll and nitrogen content. The experiment with leaves in different water stress was conducted. The statistical test result indicated that the determination of water content in leaf could be successfully performed by VIS/NIR spectroscopy combined with chemometrics method. The performances of different pretreatment methods were compared. The model with best performance was obtained from the first derivative spectra. In order to make the calibration model more parsimonious and stable, a hybrid wavelength selection method was proposed to extract the efficient feature wavelength. Under the optimal condition, an RMSEP of 0.73% with 25 variables was obtained for water content prediction using extern validation. The conclusions presented could lead to the development of portable instrument for synchronous detecting water content and other biochemical parameters rapidly and nondestructively.
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