期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 130, 期 -, 页码 440-446出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2014.04.054
关键词
Sinomenine; Lysozyme; Fluorescence quenching; Urea; Three-dimensional fluorescence; Metal ions
类别
资金
- National Natural Science Foundation of China [21372112]
- Natural Science Foundation of Luoyang Normal University [10001017]
The interaction between sinomenine and Lysozyme (Lys) in aqueous solution has been systemically investigated by fluorescence spectroscopic techniques at pH 7.4. The quenching rate constants and binding constants calculated indicated the static quenching mechanism and medium binding force. The effect of sinomenine on the conformation of Lys was analyzed using synchronous fluorescence and three-dimensional (3D) fluorescence. In addition, influence of pH on the binding of sinomenine to Lys was investigated and the binding ability of the drug to Lys deceased under other pH conditions (pH 9.0, 3.5, and 1.9) as compared with that at pH 7.4. As compared with the binding ability of sinomenine to native Lys, that of sinomenine to denatured Lys deceases dramatically. Furthermore, the effect of many metal ions on the binding constant of sinomenine with Lys was investigated. (C) 2014 Elsevier B.V. All rights reserved.
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