期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 132, 期 -, 页码 84-90出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2014.04.160
关键词
G-quadruplex; Telomere; Ruthenium complex; Indoloquinoline
类别
资金
- Specialized Research Fund for the Doctoral Program of Higher Education, China [20104420120006]
- National Science Foundation for the Young of China [21101032]
- 211 Key Program of Guangdong, China
Indoloquinoline and its derivatives have been reported to be a kind of efficient G-quadruplex binder and have been found to interact preferentially to intramolecular G-quadruplex and inhibit telomerase activity in human K562 cells and SW620 cells. In contrast to indoloquinoline derivatives, much less is known about the metal complex based on indoloquinoline or its derivative. In this report, we studied the interaction of ruthenium complex [Ru(bpy)(2)(itatp)](2+) containing indoloquinoline moiety with human telomeric G-quadruplex DNA (Telo22) and c-myc G-quadruplex DNA (Pu27) by UV-visible (UV-Vis), fluorescence spectroscopy, fluorescent intercalator displacement (FID), thermal denaturation studies and CD spectroscopy. The results suggest that [Ru(bpy)(2)(itatp)](2+) displays a strong pi-pi stacking interaction with human telomeric G-quadruplex with a high binding constant (similar to 10(7) M-1), but just exhibits moderate binding affinity to c-myc G-quadruplex, thus showing significant selectivity to human telomeric G-quadruplex. The CD titration results indicate that [Ru(bpy)(2)(itatp)](2+) could effectively convert Telo22 into antiparallel G-quadruplex conformation, while in the c-myc G-quadruplex case, instead of promoting Pu27 to fold into G-quadruplex, [Ru(bpy)(2)(itatp)](2+) destroys the parallel G-quadruplex structure of Pu27. (C) 2014 Elsevier B.V. All rights reserved.
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