期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 113, 期 -, 页码 154-158出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2013.04.019
关键词
Melamine; Spectrophotometry; Mannich reaction; 1-Pyrene carboxaldehyde; 4-Hydroxyacetophenone; Liquid milk
类别
资金
- Rajamangala University of Technology Phra Nakhon (RMUTP)
A new and simple spectrophotometric method has been developed and validated for measuring the contamination of melamine in different milk products. The method is based upon measuring the absorption of the complex formed from melamine, 4-hydroxyacetophenone (Hap) and 1-pyrene carboxaldehyde (Pcd), which was adapted from the Mannich reaction. Quantitative analysis was done at a wavelength of 236 nm within a few minutes. The reaction was optimized by focusing on both obtaining high performance of the method and to concern the volatility and toxicity of used reagents. This method provided a linear dynamic range, limit of detection and limit of quantification of 0.100-3.78, 0.08 and 0.14 mg L-1, respectively. The relative standard deviation (RSD) was 3.6% (n = 10). The recoveries of melamine spiked liquid milk samples, with melamine concentrations of 0.63, 1.26, 1.89 and 2.52 mg L-1, were 87.7 +/- 3.7%, 91.1 +/- 8.8%, 89.2 +/- 4.4% and 90.6 +/- 3.6% (n = 3), respectively. (C) 2013 Elsevier B.V. All rights reserved.
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