期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 97, 期 -, 页码 521-525出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2012.06.040
关键词
Luteolin; Calf thymus DNA; UV-spectroscopy; Fluorescence spectroscopy; Binding constants; Viscosity measurements
类别
资金
- National Natural Science Foundation of China [21005047]
- Shandong Natural Science Foundation [ZR2010BL013]
- Excellent Middle-age and Young Scientists Research Award Foundation of Shandong Province [BS2010SW012]
- Instruments Upgrading Special Foundation of Shandong Province [2011SJGZ23]
The interaction of luteolin with calf thymus deoxyribonucleic acid (ctDNA) under physiological conditions (Tris-HCl buffer solutions, pH 7.4) was studied by UV-Vis spectroscopy, fluorescence spectroscopy and viscosity measurement method, respectively. The results indicated that a complex of luteolin with ctDNA can be formed. Spectroscopic techniques together with viscosity determination provided evidences of intercalation mode of binding for the interaction between luteolin and ctDNA. The binding constant of luteolin to DNA calculated based on UV-Vis spectroscopy data was found to be 4.52 x 10(4) L mol(-1) at 310 K. The thermodynamic parameters of the complex were calculated by a double reciprocal method: Delta H-r(m)s = -8.9 x 10(3) J mol(-1) Delta S-r(m)s = 60.5 JK(-1) mol(-1) and Delta(r)G(m)(s) = -2.76 x 10(4) J mol(-1) (310 K). The interacting forces between luteolin and DNA mainly included hydrophobic interactions and hydrogen bonds. The acridine orange displacement studies revealed that luteolin had significant effect for acridine orange bounded on DNA, which was indicative of intercalation binding. (C) 2012 Elsevier B.V. All rights reserved.
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