期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 97, 期 -, 页码 119-124出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2012.05.060
关键词
Amphiphilic drug; Serum albumin; Fluorescence; Circular dichroism
类别
资金
- UGC-DAE CSR [CSR/AO/MUM/CRS-M-147/09/464]
- CSIR-SRF [09/112(0462)/2K11-EMR-I]
To know the interaction of amphiphilic drugs nortriptyline hydrochloride (NOT) and promazine hydrochloride (PMZ) with serum albumins (i.e., human serum albumin (HSA) and bovine serum albumin (BSA)), techniques of UV-visible, fluorescence, and circular dichroism (CD) spectroscopies are used. The binding affinity is more in case of PMZ with both the serum albumins. The quenching rate constant (k(q)) values suggest a static quenching process for all the drug-serum albumin interactions. The UV-visible results show that the change in protein conformation of PMZ-serum albumin interactions are more prominent as compared to NOT-serum albumin interactions. The CD results also explain the conformational changes in the serum albumins on binding with the drugs. The increment in %alpha-helical structure is slightly more for drug-BSA complexes as compared to drug-HSA complexes. (C) 2012 Elsevier B.V. All rights reserved.
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