Coordination study of recombinant human-like collagen and zinc (II)

In the present investigation, the complex of recombinant human-like collagen (r-HLC) with zinc (II) has been synthesized in aqueous solution and was analyzed by UV-vis spectroscopy, FTIR spectroscopy, circular dichroism (CD) spectroscopy. thermo gravimetric (TG) and differential scanning calorimetry (DSC) analysis. It can be concluded from UV-vis spectra that there exists interaction between r-HLC and zinc, and the complex is a new chemical compound different from pure r-HLC. In the complex of Zn, recombinant human-like collagen acts as ligand, linking the zinc ion via both groups of C=O and N-H. Besides, the results of TG and DSC confirm that the complex was significantly different from ligand, and the former is more thermally stable in comparison with the latter. The results obtained from the current investigation are of crucial importance to understand the r-HLC-Zn complex and provide theoretical evidence for the further study. (C) 2011 Elsevier B.V. All rights reserved.