期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 77, 期 3, 页码 605-611出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2010.06.023
关键词
Hematoporphyrin monomethyl ether (HMME); Resonance light scattering (RLS); ctDNA; Gemini surfactant; Interaction
类别
资金
- Guangdong Science and Technology Department [2006B35630009]
- Science Foundation of Shantou University [YR09009]
The interaction between photosensitizer anticancer drug hematoporphyrin monomethyl ether (HMME) and ctDNA has been studied based on the decreased resonance light scattering (RLS) phenomenon. The RLS, UV-vis and fluorescence spectra characteristics of the HMME-ctDNA system were investigated. Besides, the phosphodiesters quaternary ammonium salt (PQAS), a kind of new gemini surfactant synthesized recently, was used to determine anticancer drug HMME based on the increasing RLS intensity. Under the optimum assay conditions, the enhanced RLS intensity was proportional to the concentration of HMME. The linear range was 0.8-8.411 mu g mL(-1), with correlation coefficient R-2 = 0.9913. The detection limit was 0.014 mu g mL(-1). The human serum samples and urine samples were determined satisfactorily, which proved that this method was reliable and applicable in the determination of HMME in body fluid. The presented method was simple, sensitive and straightforward and could be a significant method in clinical analysis. (C) 2010 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据