期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 76, 期 2, 页码 155-160出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2010.03.006
关键词
Nickel ion; Bovine hemoglobin; Multi-spectroscopic techniques; Toxicity evaluation
类别
资金
- NSFC [20875055]
- Ministry of Education of China [708058]
- Foundation for Middle Young Scientists and Key Science-Technology Project in Shandong Province [2007B508005, 2008GG10006012]
- [NCET-06-0582]
The toxic interaction between Ni2+ and bovine hemoglobin (BHb) was investigated using fluorescence spectroscopy, synchronous fluorescence spectroscopy, ultraviolet-visible (UV-vis) absorption spectroscopy and circular dichroism spectroscopy (CD) under simulated physiological conditions. The experimental results showed that both dynamic and static quenching occurred simultaneously when Ni2+ quenched the fluorescence of BHb. The binding site number n. apparent binding constant K-d and corresponding thermodynamic parameters were measured at different temperatures. There was formation of Ni-BHb complex, but the binding between Ni2+ and BHb was not strong. The process of the formation of Ni-BHb complex was a spontaneous interaction procedure in which electrostatic interaction played a major role. In addition, UV-vis and CD results showed that the addition of Ni2+ changed the conformation of BHb. (C) 2010 Elsevier B.V. All rights reserved.
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