期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 75, 期 5, 页码 1617-1623出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2010.02.030
关键词
CdSe QDs; beta-Cyclodextrin; Phenanthroline; DNA
类别
资金
- National Natural Science Foundation of China [20975041]
- Natural Science Foundation of Guangdong Province [7005803]
- MOE Key laboratory of Laser Life Science, South China Normal University
The CdSe quantum dots (QDs) modified by mercapto-beta-cyclodextrin (CD) were synthesized and characterized by transmission electron microscopy, powder X-ray diffraction, excitation and emission spectra, and fluorescence lifetime. When lambda(ex) = 370 nm, the fluorescence peak of CdSe/CD QDs is at 525 nm. Phenanthroline (Phen) is able to quench their fluorescence, which can be recovered by the addition of DNA. The quenching and restoration of fluorescence intensity were found to be linearly proportional to the amount of Phen and DNA, respectively. The variation of the fluorescence intensity of the CdSe/CD QDs-Phen system was studied, and it was demonstrated to result from a static mechanism due to the formation of a Phen inclusion complex with the CdSe QDs modified by mercapto-beta-cyclodextrin. The fluorescence recovery was due to the binding of DNA with Phen in the inclusion complex, leading to the freeing of the CdSe/CD QDs. The binding constants and sizes of the binding sites of the Phen-DNA interaction were calculated to be 1.33 x 10(7) mol(-1) L and 10.79 bp. (C) 2010 Elsevier B.V. All rights reserved.
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