期刊
SOMATOSENSORY AND MOTOR RESEARCH
卷 27, 期 4, 页码 131-148出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/08990220.2010.513111
关键词
head fixation; psychophysics; operant conditioning; whisker; rodent
资金
- German Ministry for Education and Research [0811457]
- Deutsche Forschungsgemeinschaft [SFB 550-B11, SCHW577/7-1, SCHW577/9-1, SCHW577/10-1]
- Deutsche Forschungsgemeinschaft (Graduiertenkolleg Kognitive Neurobiologie)
- Studienstiftung des deutschen Volkes
- IZKF Medical School University Tubingen
- Hertie Foundation
- Hermann and Lilly Schilling Foundation
This paper describes experimental techniques with head-fixed, operantly conditioned rodents that allow the control of stimulus presentation and tracking of motor output at hitherto unprecedented levels of spatio-temporal precision. Experimental procedures for the surgery and behavioral training are presented. We place particular emphasis on potential pitfalls using these procedures in order to assist investigators who intend to engage in this type of experiment. We argue that head-fixed rodent models, by allowing the combination of methodologies from molecular manipulations, intracellular electrophysiology, and imaging to behavioral measurements, will be instrumental in combining insights into the functional neuronal organization at different levels of observation. Provided viable behavioral methods are implemented, model systems based on rodents will be complementary to current primate models-the latter providing highest comparability with the human brain, while the former offer hugely advanced methodologies on the lower levels of organization, for example, genetic alterations, intracellular electrophysiology, and imaging.
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