期刊
SOIL SCIENCE AND PLANT NUTRITION
卷 57, 期 2, 页码 272-278出版社
WILEY-BLACKWELL
DOI: 10.1080/00380768.2011.576398
关键词
Arabidopsis thaliana; antimony; arsenic; phytochelatin
资金
- Japanese Society for the Promotion of Science
- Ministry of Agriculture, Forestry, and Fisheries of Japan [IPG-0005]
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Grants-in-Aid for Scientific Research [21228002] Funding Source: KAKEN
To identify genes involved in arsenite [As(III)] tolerance, we screened for As(III)-sensitive mutants using ethyl methanesulfonate (EMS)-mutagenized seeds of Arabidopsis thaliana. One mutant with high sensitivity to As(III) was isolated from the screening. It had a mutation in Glu52 of phytochelatin synthase 1 (AtPCS1), and introduction of the wild-type AtPCS1 gene rescued the phenotype, confirming that the mutant represented a new allele of atpcs1 (termed as cad1-7). The expression of AtPCS1 from cad1-7 and cad1-3, a second allele of atpcs1, complemented As-sensitive yeast, but not plants. Our study suggests that AtPCS1 activity is differentially regulated between plants and yeast.
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