期刊
SOIL BIOLOGY & BIOCHEMISTRY
卷 76, 期 -, 页码 53-56出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2014.05.010
关键词
Archaea; Bacteria; CARD-FISH; High-throughput sequencing; Soil prokaryotic community; Tundra ecosystem
类别
资金
- Research Fellowships of Japan Society for the Promotion of Science (JSPS) [23-586, 24-3240]
- JSPS [26840145]
- Grants-in-Aid for Scientific Research [26840145] Funding Source: KAKEN
In the present study, we perform the first direct analysis on how the composition of the prokaryotic soil community differs depending on whether high-throughput sequencing or fluorescent in situ hybridization (FISH) coupled with catalyzed reporter deposition (CARD) is used. Soil samples were collected along short (<3 m) tundra vegetation gradients from Northern Sweden. Relative abundances of Acid-obacteria and Bacteroidetes estimated by the high-throughput sequencing were higher than those estimated by CARD-FISH, while relative abundances of Archaea and alpha-Proteobacteria estimated by high-throughput sequencing were lower than those estimated by CARD-FISH. The results indicated that the high-throughput sequencing overestimates/underestimates the relative abundance of some microbial taxa if we assume that CARD-FISH can provide potentially more quantitative data. Great caution should be taken when interpreting data generated by molecular technologies (both of high-throughput sequencing and CARD-FISH), and supports by multiple approaches are necessary to make a robust conclusion. (C) 2014 Elsevier Ltd. All rights reserved.
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