期刊
SOIL BIOLOGY & BIOCHEMISTRY
卷 57, 期 -, 页码 943-945出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2012.09.001
关键词
Molecular gut content analysis; Predator-prey interactions; Araneae; Chilopoda; Collembola; Diptera; Gamasina; Oribatida; Isopoda; Staphylinidae; 18S rDNA; COI
类别
资金
- DFG Priority Program 1374 Infrastructure Biodiversity Exploratories [SCHE 376/22-2]
Molecular gut content analysis provides a highly specific and sensitive tool to examine the diet of soil invertebrates. Here, we present new polymerase chain reaction (PCR) assays for the detection of twelve prey taxa common in Central European forest soils. The assays target five species of collembolans as well as dipterans, gamasid and oribatid mites, lithobiid centipedes, spiders, staphylinid beetles and woodlice at the group level, amplifying 123-299 bp long DNA fragments. Cross-reactivity tests against 119 soil invertebrate taxa confirm their specificity. These new PCR assays were found to be highly sensitive, revealing the consumption of five different prey taxa in field-collected centipedes. Thus they provide a ready-to-use approach for unravelling trophic interactions among soil arthropods. (C) 2012 Elsevier Ltd. All rights reserved.
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