期刊
SOIL BIOLOGY & BIOCHEMISTRY
卷 43, 期 7, 页码 1450-1455出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2011.03.012
关键词
Verrucomicrobia; Spartobacteria; Bacteria; Relative abundance; Ecological dominance; Primer bias; Barcoded pyrosequencing; 16S rRNA genes; Oligotroph; Subsurface horizon
类别
资金
- Bill and Melinda Gates Foundation
- Crohn's and Colitis Foundation of America
- National Institutes of Health
- National Science Foundation
- U.S. Department of Agriculture
- Division Of Environmental Biology
- Direct For Biological Sciences [953331] Funding Source: National Science Foundation
Verrucomicrobia are ubiquitous in soil, but members of this bacterial phylum are thought to be present at low frequency in soil, with few studies focusing specifically on verrucomicrobial abundance, diversity, and distribution. Here we used barcoded pyrosequencing to analyze verrucomicrobial communities in surface soils collected across a range of biomes in Antarctica, Europe, and the Americas (112 samples), as well as soils collected from pits dug in a montane coniferous forest (69 samples). Data collected from surface horizons indicate that Verrucomicrobia average 23% of bacterial sequences, making them far more abundant than had been estimated. We show that this underestimation is likely due to primer bias, as many of the commonly used PCR primers appear to exclude verrucomicrobial 165 rRNA genes during amplification. Verrucomicrobia were detected in 180 out of 181 soils examined, with members of the class Spartobacteria dominating verrucomicrobial communities in nearly all biomes and soil depths. The relative abundance of Verrucomicrobia was highest in grasslands and in subsurface soil horizons, where they were often the dominant bacterial phylum. Although their ecology remains poorly understood, Verrucomicrobia appear to be dominant in many soil bacterial communities across the globe, making additional research on their ecology clearly necessary. (C) 2011 Elsevier Ltd. All rights reserved.
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