期刊
SOIL BIOLOGY & BIOCHEMISTRY
卷 43, 期 10, 页码 2023-2031出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2011.05.018
关键词
Stable isotopes; Carbon; Soil microbial biomass; C metabolism; Pentose phosphate pathway; Glycolysis; Krebs cycle; Modeling; Temperature
类别
资金
- National Science Foundation [0747397, DBI-0723250]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0934398] Funding Source: National Science Foundation
- Emerging Frontiers
- Direct For Biological Sciences [0747397] Funding Source: National Science Foundation
We used metabolic tracers and modeling to analyze the response of soil metabolism to a sudden change in temperature from 4 to 20 degrees C. We hypothesized that intact soil microbial communities would exhibit shifts in pentose phosphate pathway and glycolysis activity in the same way as is regularly observed for individual microorganisms in pure culture. We also hypothesized that increased maintenance respiration at higher temperature would result in greater energy production and reduced carbon use efficiency (CUE). Two hours after temperature increase, respiration increased almost 10-fold. Although all metabolic processes were increased, the relative activity of metabolic processes, biosynthesis, and energy production changed. Pentose phosphate pathway was reduced (17-20%), while activities of specific steps in glycolysis (51%) and Krebs cycle (7-13%) were increased. In contrast, only small but significant changes in biosynthesis (+2%), ATP production (-3%) and CUE (+2%) were observed. In a second experiment, we compared the metabolic responses to temperature increases in soils from high and low elevation. The shift in activity from pentose phosphate pathway to glycolysis with higher temperature was confirmed in both soils, but the responses of Krebs cycle, biosynthesis. ATP production, and CUE were site dependent. Our results indicate that 1) in response to temperature, communities behave biochemically similarly to single species and, 2) our understanding of temperature effects on CUE, energy production and use for maintenance and growth processes is still incomplete. (C) 2011 Elsevier Ltd. All rights reserved.
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