Nitrogen fertilization inhibits soil microbial respiration regardless of the form of nitrogen applied

We tested how amendments of different forms of nitrogen (N) affect microbial respiration rates by adding six different forms of N (NH4NO3, (NH2)(2)CO (urea), KNO3, NH4CI, (NH4)(2)SO4, Ca(NO3)(2)) to three distinct soils. All inorganic N forms led to a net reduction in microbial respiration, and the magnitude of the observed response (up to 60 % reduction) was consistent across all soils and negatively correlated with N concentration. Urea also reduced respiration rates in nearly all cases, but the effect was attenuated by the associated input of labile organic carbon. We observed decreases in respiration regardless of soil type, the specific N counter ion, N added as NF4+ or NO3-, or the effects of N form on soil pH, suggesting that decreases in respiration rates were mainly a direct result of the increase in soil N availability, rather than indirect effects caused by the form of N added. (c) 2010 Elsevier Ltd. All rights reserved.