Carbon partitioning in ectomycorrhizal Scots pine seedlings

The complete carbon budget and the turnover rate of assimilated carbon of ectomycorrhizal Scots pine seedlings growing on natural humus were determined in microcosm conditions. The main aim was to improve understanding of the partitioning of the assimilated carbohydrates within seedlings associated with multiple ectomycorrhizal fungi, and to discover carbon dynamics of the mycorrhizosphere. Plant photosynthesis and below-ground respiration were measured in order to obtain the actual carbon assimilation and respiration rates at the time of measurements. Soon after the photosynthesis and respiration rate measurements the seedlings were pulse-labeled with (CO2)-C-14 to follow carbon allocation to different plant, fungal and soil compartments and rhizosphere respiration. Long-term carbon allocation during the entire life span of the seedlings was estimated by measuring plant and mycorrhizal root-tip biomass. The ectomycorrhizal community was analyzed using morphotyping and ITS-sequencing. The C-14 label was detected in rhizosphere respiration after 12 h and it peaked between 36 and 60 h after labeling. More than half of the assimilated carbon was allocated below-ground as biomass or respiration and higher mycorrhizal biomass increased the below-ground carbon turnover. The presence of Suillus variegatus affected the plant carbon balance in several ways. When S. variegatus was present, the below-ground respiration increased and this carbon loss was compensated by higher photosynthetic activity. Other fungal species did not differ between each other in their effects on carbon balance. Our findings indicate that some root-associated mycorrhizal fungal symbionts can significantly alter plant CO2 exchange, biomass distribution, and the allocation of recently photosynthesized plant-derived carbon. (C) 2010 Elsevier Ltd. All rights reserved.