期刊
ADVANCED HEALTHCARE MATERIALS
卷 4, 期 9, 页码 1332-1338出版社
WILEY
DOI: 10.1002/adhm.201500132
关键词
gene expression; nucleic acids; RT-PCR; sol-gel; storage
资金
- ETH Zurich (Institute for Chemical and Bioengineering)
- EU-ITN network Mag(net)icFun [PITN-GA-2012-290248]
As a consequence of the latest revolutionary discoveries on its functions, RNA is certainly the hottest topic at the moment, being an exceptional tool in biology as well as in medicine. For the various applications, a proper RNA storage is required to prevent the degradation of this extremely unstable molecule. Here a novel freezing-free RNA storage strategy is presented, based on its encapsulation in silica spheres. The silica microcapsules protect the RNA by providing a water-free environment. In this way RNA can be safely stored for prolonged periods of time at ambient and elevated temperatures, maintaining its original integrity, as proved by gel-electrophoresis, capillary electrophoresis, and real-time reverse transcription-polymerase chain reaction (RT-qPCR). The RNA degradation rate at 65 degrees C in silica microcapsules is approximately ten times smaller in comparison to dry RNA samples or to samples stored in RNAstable matrix, a commercially available product. Moreover, RNA half-life at 65 degrees C is nearly identical to that of DNA within the silica microcapsules. Samples intended for use in gene expression are compatible with further analysis (RT-qPCR, Sanger sequencing). The novel storage technology permits to safely handle, store, and transport RNA samples, avoiding the expensive shipments and the problems of space presented by freezing-based strategies.
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