期刊
SOFT MATTER
卷 10, 期 8, 页码 1199-1213出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3sm52496e
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资金
- National Program on Key Basic Research Project of China (973 Program) [2013CB328900]
- National Natural Science Foundation of China [21001077, 20321061]
- Program for Changjiang Scholars in China
A series of novel aliphatic polyesters with azido functional groups were synthesized via the direct lipasecatalyzed polycondensation of dialkyl diester, diol and 2-azido-1,3-propanediol (azido glycerol) using immobilized lipase B from Candida antarctica (CALB). The effects of polymerization conditions including reaction time, temperature, enzyme amount, substrates and monomer feed ratio on the molecular weights of the products were studied. The polyesters with pendant azido groups were characterized by H-1 NMR, C-13 NMR, 2D NMR, FTIR, GPC and DSC. Alkyne end-functionalized poly(ethylene glycol) containing a cleavable acetal group was then grafted onto the polyester backbone by copper-catalyzed azide-alkyne cycloaddition (CuAAC, click chemistry). Using fluorescence spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy (TEM), these amphiphilic graft copolymers were found to readily self-assemble into nanosized micelles in aqueous solution with critical micelle concentrations between 0.70 and 1.97 mg L-1, and micelle sizes from 20-70 nm. The degradation of these polymers under acidic conditions was investigated by GPC and H-1 NMR spectroscopy. Cell cytotoxicity tests indicated that the micelles had no apparent cytotoxicity to Bel-7402 cells, suggesting their potential as carriers for controlled drug delivery.
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