期刊
SOFT MATTER
卷 8, 期 16, 页码 4390-4395出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2sm07227k
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资金
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
- Foundation Hattori-Hokokai
- MEXT
- Grants-in-Aid for Scientific Research [22018014, 23685050, 23350054] Funding Source: KAKEN
A single polymer chain was directly observed via a super-resolution optical imaging technique, photoactivated localization microscopy (PALM). A hydrophobic rhodamine spiroamide derivative enabled the PALM observation of synthetic polymers. The conformation of a single chain of poly(butyl methacrylate) was investigated with a spatial resolution of 15 nm. The distribution functions of the chain conformation were directly obtained from the high-resolution image of individual chains. Moreover, the three-dimensional structure of individual chains was successfully observed with a lateral spatial resolution of 20 nm and a depth resolution of 40 nm.
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