期刊
ACS SYNTHETIC BIOLOGY
卷 5, 期 1, 页码 8-14出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.5b00094
关键词
cyanobacteria; microfluidics; dynamics; Synechocystis; Synechococcus; Chlorella
资金
- National Institutes of Health and General Medicine [R01GM69811, GM100116]
- UCSD Chancellor's Collaboratories Program for Interdisciplinary Scholarship
- American Cancer Society Postdoctoral Fellowship [PF-12-262-01-MPC]
Culturing cells in microfluidic lab-on-a-chip devices for time lapse microscopy has become a valuable tool for studying the dynamics of biological systems. Although microfluidic technology has been applied to culturing and monitoring a diverse range of bacterial and eukaryotic species, cyanobacteria and eukaryotic microalgae present several challenges that have made them difficult to culture in a microfluidic setting. Here, we present a customizable device for the long-term culturing and imaging of three well characterized strains of cyanobacteria and microalgae. This platform has several advantages over agarose pads and demonstrates great potential for obtaining high quality, single-cell gene expression data of cyanobacteria and algae in precisely controlled, dynamic environments over long time periods.
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