We report the creation of biopolymer capsules that show a colorimetric response to changes in pH and temperature. Polymerized diacetylenic (PDA) vesicles are embedded within capsules formed by complexing the cationic biopolymer, chitosan, with an anionic surfactant. The PDA vesicles impart their colorimetric properties to the capsules while remaining embedded within the capsule lumen. Accordingly, the capsules show a blue color at low pH (similar to 6) or temperature (similar to 25 degrees C) whereas the color changes to purple at medium pH (similar to 8) or temperature (similar to 45 degrees C) and finally to red at high pH (similar to 10) or temperature (similar to 60 degrees C). These capsule-based sensors are easy to prepare, low-cost, and can be easily tailored for various applications.
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