期刊
ACS SYNTHETIC BIOLOGY
卷 5, 期 7, 页码 554-560出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.5b00144
关键词
optogenetics; LOV domain; caged peptide; peroxisome; trafficking
资金
- National Institutes of Health [GM100225, DK081584]
- University of Colorado
The blue-light-responsive LOV2 domain of Avena sativa phototropinl (AsLOV2) has been used to regulate activity and binding of diverse protein targets with light. Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import. We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light. This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
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