期刊
ACS SYNTHETIC BIOLOGY
卷 5, 期 1, 页码 99-103出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.5b00124
关键词
assembly; modular; part library; Type IIS; multiplex
资金
- NSF [1253856]
- Div Of Molecular and Cellular Bioscience [1253856] Funding Source: National Science Foundation
Multipart and modular DNA part libraries and assembly standards have become common tools in synthetic biology since the publication of the Gibson and Golden Gate assembly methods, yet no multipart modular library exists for use in bacterial systems. Building upon the existing MoClo assembly framework, we have developed a publicly available collection of modular DNA parts and enhanced MoClo protocols to enable rapid one-pot, multipart assembly, combinatorial design, and expression tuning in Escherichia coli. The Cross-disciplinary Integration of Design Automation Research lab (CIDAR) MoClo Library is openly available and contains promoters, ribosomal binding sites, coding sequence, terminators, vectors, and a set of fluorescent control plasmids. Optimized protocols reduce reaction time and cost by >80% from that of previously published protocols.
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