期刊
SMALL
卷 14, 期 39, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201802630
关键词
airflow-assisted; bioprinting; heterogeneous; microenvironments; microspheroids; organoids; spiral
类别
资金
- National Nature Science Foundation of China [51622510, U1609207]
- Science Fund for Creative Research Groups of the National Natural Science Foundation of China [51521064]
- Nature Science Foundation of Zhejiang Province, China [LR17E050001]
- key Research and Development Program of Zhejiang Province [2017C01063]
- Natural Sciences Foundation of China [81630065]
Hydrogel microspheroids are widely used in tissue engineering, such as injection therapy and 3D cell culture, and among which, heterogeneous microspheroids are drawing much attention as a promising tool to carry multiple cell types in separated phases. However, it is still a big challenge to fabricate heterogeneous microspheroids that can reconstruct built-up tissues' microarchitecture with excellent resolution and spatial organization in limited sizes. Here, a novel airflow-assisted 3D bioprinting method is reported, which can print versatile spiral microarchitectures inside the microspheroids, permitting one-step bioprinting of fascinating hydrogel structures, such as the spherical helix, rose, and saddle. A microfluidic nozzle is developed to improve the capability of intricate cell encapsulation with heterotypic contact. Complex structures, such as a rose, Tai chi pattern, and single cell line can be easily printed in spheroids. The theoretical model during printing is established and process parameters are systematically investigated. As a demonstration, a human multicellular organoid of spirally vascularized ossification is reconstructed with this method, which shows that it is a powerful tool to build mini tissues on microspheroids.
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