期刊
SMALL
卷 14, 期 34, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201801731
关键词
advection flows; bacteria separation; magnetic separation; magnetophoresis; microfluidics
类别
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2016R1D1A1B03931492]
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Korean government (MSIT) [NRF-2017M3A9E2062136]
- UNIST [1.180063.01]
- Ministry of Science & ICT (MSIT), Republic of Korea [2018미래선도형 특성화연구사업] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2017M3A9E2062136, 2016R1D1A1B03931492] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A major challenge to scale up a microfluidic magnetic separator for extracorporeal blood cleansing applications is to overcome low magnetic drag velocity caused by viscous blood components interfering with magnetophoresis. Therefore, there is an unmet need to develop an effective method to position magnetic particles to the area of augmented magnetic flux density gradients while retaining clinically applicable throughput. Here, a magnetophoretic cell separation device, integrated with slanted ridge-arrays in a microfluidic channel, is reported. The slanted ridges patterned in the microfluidic channels generate spiral flows along the microfluidic channel. The cells bound with magnetic particles follow trajectories of the spiral streamlines and are repeatedly transferred in a transverse direction toward the area adjacent to a ferromagnetic nickel structure, where they are exposed to a highly augmented magnetic force of 7.68 mu N that is much greater than the force (0.35 pN) at the side of the channel furthest from the nickel structure. With this approach, 91.68% +/- 2.18% of Escherichia coli (E. coli) bound with magnetic nanoparticles are successfully separated from undiluted whole blood at a flow rate of 0.6 mL h(-1) in a single microfluidic channel, whereas only 23.98% +/- 6.59% of E. coli are depleted in the conventional microfluidic device.
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