期刊
SMALL
卷 11, 期 1, 页码 119-125出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201402016
关键词
-
类别
资金
- Bundesministerium fur Bildung und Forschung (BMBF) [FKZ 0315316B]
- Bundesministerium fur Wirtschaft und Technologie (BMWi, Exist Research Transfer) [FKZ 03EFT9BW44]
Efficient use of membrane protein nanopores in ionic single-molecule sensing requires technology for the reliable formation of suspended molecular membranes densely arrayed in formats that allow high-resolution electrical recording. Here, automated formation of bimolecular lipid layers is shown using a simple process where a poly(tetrafluoroethylene)-coated magnetic bar is remotely actuated to perform a turning motion, thereby spreading phospholipid in organic solvent on a nonpolar surface containing a <1 mm(2) 4 x 4 array of apertures with embedded microelectrodes (microelectrode cavity array). Parallel and high-resolution single-molecule detection by single nanopores is demonstrated on the resulting bilayer arrays, which are shown to form by a classical but very rapid self-assembly process. The technique provides a robust and scalable solution for the problem of reliable, automated formation of multiple independent lipid bilayers in a dense microarray format, while preserving the favorable electrical properties of the microelectrode cavity array.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据