期刊
SMALL
卷 10, 期 9, 页码 1857-1865出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201303594
关键词
nanocages; fullerenes; electrochemiluminescence; bioactive materials
类别
资金
- NNSF of China [21075100, 21275119]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC2010009]
- Ministry of Education of China [708073]
- Natural Science Foundation of Chongqing City [CSTC-2010BB4121, CSTC-2011BA7003]
- Specialized Research Fund for the Doctoral Program of Higher Education [20100182110015]
- Fundamental Research Funds for the Central Universities [XDJK2012A004, XDJK2013A008]
A novel functionalized material is synthesized using surface-decorated fullerene (C-60) to encapsulate hollow and porous palladium nanocages (PdNCs), and is applied to fabricate an electrochemiluminescence (ECL) immunosensor for the detection of Streptococcus suis Serotype 2 (SS2). PdNCs with hollow interiors and porous walls are prepared using a galvanic replacement reaction between silver nanocubes and metal precursor salts. Then, C-60 reacts with l-cysteine (l-Cys) to form l-Cys functionalized C-60 (C-60-l-Cys), which has a better biocompatibility, conductivity, and hydrophilicity compared to C-60 and possesses abundant -SH groups on the surface. Because of the special interaction between -SH and PdNCs, the obtained C-60-l-Cys is adsorbed around the PdNCs to form an interesting structure with multiple spheres encapsulating the cage. The resultant functionalized material (C-60-L-Cys-PdNCs) has a high specific surface area, good electrocatalytic ability, and efficient photocatalytic activity, and is used to construct an ECL immunosensor for the detection of SS2. The ECL signal amplified strategy is performed by using the novel coreactant (C-60-l-Cys) and in situ generation of O-2 thus creating the S2O82--O-2 ECL system. As a result, a wide linear detection range of 0.1 pg mL(-1) to 100 ng mL(-1) is acquired with a relatively low detection limit of 33.3 fg mL(-1).
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