期刊
SMALL
卷 9, 期 23, 页码 4006-4016出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201300644
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资金
- nmC@LU
- Swedish Foundation for Strategic Research (SSF)
- Swedish Research Council (VR) [2007-584, 2008-6584, 2004-6793, 2010-4562]
- Crafoord Foundation [2005 1123]
- Knut and Alice Wallenberg Foundation
- DTU Center for Electron Nanoscopy (DTU CEN)
Nanowires are commonly used as tools for interfacing living cells, acting as biomolecule-delivery vectors or electrodes. It is generally assumed that the small size of the nanowires ensures a minimal cellular perturbation, yet the effects of nanowires on cell migration and proliferation remain largely unknown. Fibroblast behaviour on vertical nanowire arrays is investigated, and it is shown that cell motility and proliferation rate are reduced on nanowires. Fibroblasts cultured on long nanowires exhibit failed cell division, DNA damage, increased ROS content and respiration. Using focused ion beam milling and scanning electron microscopy, highly curved but intact nuclear membranes are observed, showing no direct contact between the nanowires and the DNA. The nanowires possibly induce cellular stress and high respiration rates, which trigger the formation of ROS, which in turn results in DNA damage. These results are important guidelines to the design and interpretation of experiments involving nanowire-based transfection and electrical characterization of living cells.
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