期刊
SMALL
卷 8, 期 2, 页码 259-264出版社
WILEY-BLACKWELL
DOI: 10.1002/smll.201101938
关键词
telomerases; gold nanoparticles; sensors; colorimetry; G-quadruplexes
类别
资金
- 973 Project [2011CB936004, 2012CB720602]
- NSFC [20831003, 90813001, 20833006, 90913007]
- CAS
Telomerase is over-expressed in over 85% of all known human tumors. This renders the enzyme a valuable biomarker for cancer diagnosis and an important therapeutic target. The most widely used telomeric repeat amplification protocol (TRAP) assay has been questioned for telomerase detection. It is reported that human telomerase activity can be visualized by using primer-modified Au nanoparticles. The working principle is based on the elongated primers conjugated to the gold nanoparticle (AuNP) surface, which can fold into a G-quadruplex to protect the AuNPs from the aggregation. The developed simple and sensitive colorimetric assay can measure telomerase activity down to 1 HeLa cell mu L-1. More importantly, this assay can be easily extended to high-throughput and automatic format. The AuNP-TS method is PCR-free and therefore avoids the amplification-related errors and becomes more reliable to evaluate telomerase activity. This assay has also been used for initial screening of telomerase inhibitors as anticancer drug agents.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据