期刊
SMALL
卷 7, 期 20, 页码 2921-2928出版社
WILEY-BLACKWELL
DOI: 10.1002/smll.201101210
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资金
- National Natural Science Foundation of China [20821063, 21075061, 21020102038]
- National Basic Research Program of China [2011CB933502]
A silver nanoparticle-hollow titanium phosphate sphere (AgNP-TiP) hybrid is successfully synthesized and used as a label for electrochemical detection of human interleukin-6 (IL-6). Hollow TiP spheres with a diameter of 430 nm and an average thickness of 40 nm are synthesized by a template approach. The AgNPs are incorporated in situ into the TiP shell via an exchange process. The as-prepared AgNP-TiP hybrid shows outstanding biocompatibility, good dispersity and solubility in water, and high silver loading properties (289.2 mg of silver in 1.0 g of TiP). These advantages make the AgNP-TiP hybrid an effective candidate as an amplification label in immunoassay systems. Herein, the as-prepared AgNP-TiP hybrid is attached to a signal antibody (Ab(2)) to produce Ab(2)-AgNP-TiP labels in the fabrication of an electrochemical immunosensor. The nanoparticle-based amplification labels, upon coupling with a magnetic sensing array, give rise to an extremely sensitive response to IL-6 in a linear range of 0.0005-10 ng mL(-1) with a detection limit of 0.1 pg mL(-1). The proposed sensor exhibits high specificity, good reproducibility, and long-term stability, and may be a promising technique for protein and DNA detection.
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