期刊
SMALL
卷 6, 期 2, 页码 270-275出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.200901204
关键词
cell membranes; cell receptors; focused ion beam milling; nanodomains; optical antennas
类别
资金
- EC-RTN-IMMUNANOMAP
- EC-NEST-BIO-LIGHT-TOUCH
- ICREA Funding Source: Custom
Optical antennas that confine and enhance electromagnetic fields in a nanometric region hold great potential for nanobioimaging and biosensing. Probe-based monopole optical antennas are fabricated to enhance fields localized to <30 nm near the antenna apex in aqueous conditions. These probes are used under appropriate excitation antenna conditions to image individual antibodies with an unprecedented resolution of 26+/-4 run and virtually no surrounding background. On intact cell membranes in physiological conditions, the obtained resolution is 30+/-6 nm. Importantly, the method allows individual proteins to be distinguished from nanodomains and the degree of clustering to be quantified by directly measuring physical size and intensity of individual fluorescent spots. Improved antenna geometries should lead to true live cell imaging below 10-nm resolution with position accuracy in the subnanometric range.
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