Aptatag-Based Multiplexed Assay for Protein Detection by Surface-Enhanced Raman Spectroscopy

Despite recent improvements, the development of highly sensitive and selective assays for multiplexed protein detection remains a challenging goal in modern proteomics.([1]) Key factors for successful multiplexed protein assays are high specificity, sensitivity, reproducibility, and large signal-to-noise (SIN) ratios. Multiplexed assays are necessary when fast recognition of target analytes in complex mixtures is required.([2]) The majority of protein detection methods have relied thus far on antibody/antigen recognition strategies.([3]) While this approach has proven effective for detecting single proteins, antibody cross-reactivity significantly limits implementation of enzyme-linked immunosorbent assay (ELISA) analogues for multiplexed detection.([4])